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. 2013 Oct 11;4(8):698–706. doi: 10.4161/viru.26413

graphic file with name viru-4-698-g4.jpg

Figure 4. Mixed effects of sagB deletion on emm expression. Relative levels of emm transcript (A) and western blot detecting the production of the M protein (B) in the following strains: 1, MGAS2221; 2, MGAS2221ΔsagB-1; 3, MGAS2221ΔsagB-2; 4, MGAS2221ΔsagB-3. The ΔsagB mutants were obtained using THY broth for GAS growth during the gene deletion process. Levels of emm mRNA were normalized to that of MGAS2221.