Figure 1. GSI enhances VCR-induced cell death in HeLa cells. HeLa cells were treated with VCR (10–20 nM) and/or GSI (10 μM) for 48 h. Control cells were treated with DMSO. (A and C) Cell viability was measured by WST-1 assays. Relative cell viability is presented as O.D at 450 nm. (B) Viable cell number was counted by trypan blue exclusion assay. Results are presented as mean ± SEM of triplicate assays. The statistical significance of differences was determined by ANOVA test; *P < 0.05; **P < 0.01; ***P < 0.001.