C57BL/6 (n=9) and A/J mice (n=7), and their MyD88-defiecient counterparts (n=7–9) were immunized twice on day 1 and day 21 with FliC plus OVA and sera collected on days 14 and 35 were analyzed for IgG1 (A), and IgG2a or IgG2c (B) responses against FliC by ELISA. C) Naïve, day 14, and day 35 sera were assessed for IgG1 anti-OVA responses by ELISA. Data is a combination of two-independent experiments with n = 3–5 per experiment. Statistical analyses were done using Mann-Whitney analysis of individual groups: *= P<0.05, ** = P<0.01.