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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Int J Obes (Lond). 2013 Aug 16;38(3):371–378. doi: 10.1038/ijo.2013.152

Figure 3. Confirmation of microarray gene expression levels of a select number of genes of interest, using quantitative real time qPCR.

Figure 3

The microarray expression levels of six fibroblast growth factors (FGF1, FGF7, FGF9, FGF10, FGF19, FGF21), the precursor gene of Irisin (Fibronectin type III domain containing 5 - FNDC5), and the transcription factor Peroxisome proliferator-activated receptor Gamma Coactivator 1-alpha (PGC1-α) were confirmed by qPCR, using SAT, VAT, and EAT isolated from 10 patients undergoing open Roux-en-Y gastric bypass. The Y-axis values represent the difference (Δ) of the Ct value of GAPDH from the Ct value of the gene of interest which was further adjusted by subtracting from 40. The horizontal dotted line on each figure represents the threshold of gene detection using the above equation [40-Δ(Ct-GAPDH Ct)], set at “30” which corresponds to a gene Ct value of, approximately, 35. Data are presented as mean ± SD. The different low-case letters above the columns indicate pairs that were statistically different from each other. The same letter indicates no statistical significance between adipose depots in terms of gene expression. Significance was set at P < 0.05 and determined by ANOVA and post-hoc analysis by the least square difference test (NS: Not significant, NA: Not applicable - below detection level).