Figure 2.

Cells with Neocentromere Show Normal Chromosome Segregation

(A) Growth curve of cells with neocentromere. Z#3 has endogenous centromere on chromosome Z (control). #BM23, #0303, #0727, #BL02, #0514, and #1024 cells, each of which has a neocentromere on chromosome Z, grew well as Z#3 cells.

(B) Time to complete mitotic progression, population of cells with misaligned chromosome, percentage of aneuploid cells, and numbers of cells in which sister chromatids of chromosome Z are prematurely separated in each neocentromere-containing cell line.

(C) Typical images of cells with either aligned or misaligned chromosome Z. Chromosome Z was detected by FISH the satellite sequence on q-arm of chromosome Z as a probe (red).

(D) A typical image of cell in which sister chromatids of chromosome Z are prematurely separated during prometaphase (right). Left shows a cohered chromosome Z (control).

(E) Immunofluorescence analysis on neocentromeres with antibodies against CENP-A, -C, -O, -E, -T, Ndc80, Aurora B, and KNL2. All tested centromere proteins were detected on all neocentromeres.

See also Figure S2.