Figure 5.

Chromatin Features in Various Neocentromeres

(A) ChIP-seq analysis with anti-H3K9me3 or anti-CENP-A in Z#3 cells. In chromosomes 1 and 2 that have repetitive centromeres, H3K9me3 was enriched around the CENP-A domain. H3K9me3 was not enriched around nonrepetitive centromeres of chromosomes Z, 5, and 27.

(B) ChIP-seq analysis with anti-H3K9me3 or anti-CENP-A in neocentromere-containing #BM23 and #0514 cells. H3K9me3 was not enriched around neocentromere region in both cell lines.

(C) ChIP-PCR analysis with antibodies against H3K4me2 and H3K36me2 around centromere regions in Z#3 (chromosome Z), #BM23 (Z neocentromere), and #0514 (Z neocentromere). In #BM23 and #0514 cells, these profiles are shown before and after neocentromere formation. Primer positions for this analysis and ChIP-seq data with anti-CENP-A antibodies in each cell line are also shown. Error bars show SEM.

See also Figure S5.