Figure 7.

Neocentromeres Are Efficiently Formed on Nontranscribed Region in Chromosome Z

(A) Examination of expression for genes, which are located on each neocentromere, in early embryos or wild-type DT40 cells. Expression was analyzed by RT-PCR. Most genes except for MAMDC2 are not expressed in DT40 cells, but expressed in early embryonic cells.

(B) Quantitative-RT-PCR analysis of MAMDC2 in wild-type DT40 or #0514 cells in which a neocentromere is formed on MAMDC2 gene locus (see Figure 3A). #0514-CAf cells are expressing CENP-A-FLAG. Expression level of MAMDC2 extremely was reduced in #0514 cells. Error bars show SD.

(C) Position of each neocentromere and native centromere on chromosome 5. Location of genes around neocentromeres is shown. Many neocentromeres are formed on transcribed genes in chromosome 5.