Fig. 4.

Representative micrographs show localisation of active Notch3 in SMCs infected with control AdEGFP (A) or AdHGF (B). (C) Western blotting show HES1 expression and β-tubulin expression as a loading control. *P < 0.01. (D) Localisation of Notch3 in VSMCs infected with AdHGF in the presence or absence of DAPT (1 μmol/L). (E) Alizarin red staining showed DAPT reduced mineralisation in VSMC cultured in osteogenic media for 21 days and (F) in SMCs infected with AdHGF and cultured in osteogenic media for 8 days (n = 3). Bar in A & B = 100 μm; D = 25 μm, E & F = 200 μm.