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. 2014 Feb 17;8:43. doi: 10.3389/fnbeh.2014.00043

Figure 1.

Figure 1

Visualization of vmPFC axon terminals in the DRN (A) An AAV vector coding for the Cre-dependent expression of the fluorescently tagged synaptic protein Synaptophysin (SynP-GFP) was injected in the vmPFC of CaMKIIa-Cre mice. Topographic distribution of vmPFC axon terminals in the DRN was visualized using confocal microscopy. (B) Distribution of vmPFC terminals as determined by SynP-GFP expression as reported in this study. Scale bar 50 μm. (C) Anterograde tracing using an AAV-GFP vector injected in the vmPFC as reported by the Allen Brain Connectivity Atlas. Picture is courtesy of the Allen Mouse Brain Atlas [Internet]. © 2012 Allen Institute for Brain Science (Seattle, WA). Available from: http://mouse.brain-map.org. Despite use of different tracing methods, the pattern of innervation revealed is strikingly similar, with sparse innervation of the midline area of the DRN, classically containing 5-HT neurons, and denser innervation of lateral portions of the DRN. Scale bar 50 μm.