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. 2014 Feb 17;8:43. doi: 10.3389/fnbeh.2014.00043

Figure 5.

Figure 5

Photoactivation of vmPFC terminals results in time-locked EPSC events in DRN GABA neurons. (A) High magnification confocal image of a biocytin-filled recorded GAD2-tdTomato neuron (red) in close contact with axon terminals from vmPFC neurons (green) anterogradely traced using AAV-CaMKIIa-ChR2-YFP. Inset displays a schematic of DRN topography in recorded slices from GAD2-tdTomato mice. Recorded neurons were purposely selected in ventrolateral subregions of the DRN that are rich in afferents from the vmPFC while the DRN was photostimulated. Aq—aqueduct. (B) Average voltage-clamp traces of spontaneous and laser evoked EPSC events. Application of 20 μM DNQX prevented laser-evoked EPSC events. Scale bar 4 pA, 3 ms. (C) Raw voltage-clamp data trace recorded from a GAD2-tdTomato neuron during pulsed photoactivation of ChR2 containing vmPFC terminals (473 nm, 10 mW, 0.5 Hz, 10 ms pulse width). Blue circles mark laser pulses. Black triangles indicate spontaneous EPSC events. Scale bar 10 pA, 0.5 s. (D) EPSC events were time-locked to a 25 Hz laser stimulation train. Scale bar 5 pA, 0.1 s. (E) Recordings from Pet1-tdTomato neurons did not display EPSC events in response to laser stimulation. Scale bar 10 pA, 0.5 s.