Table 2. Primers designed in this study.
Primer name | Nucleotide sequence (5′-3′) | Target gene | Target organism |
---|---|---|---|
aCHS-F | TCGGAAACGTCACATGCATC | CHS | A. thaliana |
aCHS-R | GTCCAGAGAAGGAGCCATGTAAG | CHS | A. thaliana |
aDFR-F | TATCACCGCGCTCTCTCCTATC | DFR | A. thaliana |
aDFR-R | GTCGTCCAAATGCACATACTGTC | DFR | A. thaliana |
btublin-F | TGAAGGAATGGACGAGATGGA | β-tublin | A. thaliana |
btublin-R | TGCAGTTGCGTCTTGGTATTG | β-tublin | A. thaliana |
CHS-F | TGGTGACAGTCGAGGAGTATCG | CHS | P. hybrida |
CHS-R | TAGGTGTGGCTGTTCCAATGG | CHS | P. hybrida |
DFRA-F | CCGAAGTGCTGAAGACAATGG | DFR | P. hybrida |
DFRA-R | TGCATTCTCTTTGCCACTTGC | DFR | P. hybrida |