Table 3.

Virtual restriction mapping of 3.56 Kb unit of DYZ1 array from twin pairs (blood DNA) using restriction mapper software

Sr. no.	Restriction site	Sequence	Overhang	Restriction site frequency
				MZT1a	MZT1b	MZT2a	MZT2b	MZT3a	MZT3b
1	Cac8I	GCNNGC	5’	1	1	1	1	2	1
2	BseYI	CCCAGC	5’	1	1	1	1	1	1
3	Bsp1407I	TGTACA	5’	1	1	1	1	0	0
4	BspHI	TCATGA	3’	1	1	1	1	0	0
5	ClaI	ATCGAT	3’	1	1	1	1	1	1
6	DdeI	CTNAG	blunt	1	1	0	1	0	0
7	EcoRI	GAATTC	5’	1	1	1	1	2	1
8	ScrFI	CCNGG	3’	1	1	1	1	1	1
9	VspI	ATTAAT	blunt	1	1	1	1	1	1
10	BseMII	CTCAG	5’	1	1	0	1	0	0
11	BsgI	GTGCAG	3’	1	1	1	1	0	0
12	BtsI	GCAGTG	3’	1	1	1	1	0	0
13	Eco57I	CTGAAG	3’	1	1	1	1	1	1
14	GsuI	CTGGAG	3’	1	1	1	1	0	0
15	Hpy188I	TCNGA	3’	1	1	1	1	0	0
16	MnlI	CCTC	3’	1	1	2	2	0	0
17	PflMI	CCANNNNNTGG	3’	1	1	1	1	1	1
18	SduI	GDGCHC	blunt	1	1	1	1	0	0
19	BsaBI	GATNNNNATC	blunt	0	0	1	0	0	0
20	TaqII	GACCGA	3’	0	0	2	0	0	0
21	MfeI	CAATTG	5’	0	0	0	2	0	0
22	DpnI	GATC	5’	2	2	1	1	1	1
23	FokI	GGATG	3’	2	2	2	2	2	2
24	MboI	GATC	5’	2	2	1	1	1	1
25	ArsI	GACNNNNNNTTYG	5’	2	2	0	2	2	2
26	BdaI	TGANNNNNNTCA	5’	2	2	2	2	2	2
27	CspCI	CAANNNNNGTGG	5’	2	0	0	0	0	0
28	Eco57MI	CTGRAG	5’	2	2	2	2	1	1
29	SetI	ASST	5’	2	2	2	2	2	1
30	TspRI	CASTG	5’	2	2	2	2	1	1
31	ApoI	RAATTY	3’	3	3	3	3	4	2
32	AsuII	TTCGAA	3’	3	3	3	3	3	4
33	Eco31I	GGTCTC	blunt	3	3	3	3	3	3
34	TatI	WGTACW	5’	3	3	3	4	1	1
35	MboII	GAAGA	3’	3	4	4	3	3	4
36	CviJI	RGCY	blunt	4	4	4	4	3	3
37	MslI	CAYNNNNRTG	5’	4	4	4	4	3	3
38	BccI	CCATC	3’	4	4	5	4	4	5
39	MaeI	CTAG	3’	4	4	4	4	3	4
40	SfaNI	GCATC	3’	4	4	3	4	2	2
41	AlfI	GCANNNNNNTGC	3’	4	4	2	4	2	2
42	TstI	CACNNNNNNTCC	3’	4	4	4	5	4	4
43	RsaI	GTAC	3’	5	5	5	5	2	3
44	BsmAI	GTCTC	3’	5	5	5	5	5	5
45	BstXI	CCANNNNNNTGG	blunt	5	4	5	5	5	5
46	XmnI	GAANNNNTTC	5’	6	6	6	6	5	6
47	MseI	TTAA	3’	6	6	6	5	6	6
48	NlaIII	CATG	5’	7	7	9	7	5	4
49	BcgI	CGANNNNNNTGC	5’	14	14	18	16	10	12
50	BsrDI	GCAATG	5’	15	14	14	16	11	11
51	BsrI	ACTGG	5’	19	18	19	18	12	14
52	TspGWI	ACGGA	5’	19	19	21	19	16	16
53	BsmI	GAATGC	5’	21	20	22	22	13	17
54	AgsI	TTSAA	5’	22	22	24	25	24	20
55	FaiI	YATR	3’	23	23	25	23	17	16
56	PleI	GAGTC	3’	23	23	24	23	19	18
57	TspDTI	ATGAA	blunt	25	25	23	24	18	17
58	TspEI	AATT	5’	34	32	36	34	32	29
59	TfiI	GAWTC	3’	53	53	51	54	48	48
60	TaqI	TCGA	blunt	66	67	61	67	54	55
61	HinfI	GANTC	5’	76	76	75	77	67	66

The differences are highlighted in bold.