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. 2014 Feb 4;5:3224. doi: 10.1038/ncomms4224

Figure 6. NRDc regulates transcription of PGC-1α and its downstream target genes.

Figure 6

(a) mRNA levels of thermogenic genes in primary brown adipocytes transduced with lentivirus vector expressing either a control miR or miRs targeted to Nrd1. n=6. (b) Protein levels of NRDc and UCP1 in primary brown adipocytes described in a. Full blots are presented in Supplementary Fig. 9. (c) mRNA levels of PGC-1α and UCP1 in mature immortalized brown adipocytes derived from Nrd1+/− and Nrd1−/− BAT in the presence or absence of forskolin treatment for 2 h. n=6. (d) Transcriptional activity of ppargc1α promoter-driven reporter gene in response to NRDc expression in the presence or absence of forskolin treatment in COS7 cells. n=5. (e) mRNA levels of PGC-1α target genes in primary brown adipocytes transduced with lentivirus vector as described in a. n=6. (f) mRNA levels of PGC-1α target genes in BAT of 12-week-old Nrd1+/+ and Nrd1−/− mice. n=6. All data represent means±s.e.m. *P<0.05, **P<0.005, ***P<0.001, NS, not significant (two-tailed Student’s t-test).