Fig. 2.

Analysis of binding of E18 and E19 to EV71 viral proteins by immunoblot and indirect ELISA. (A) Mock- (M) and EV71-infected (V) cell lysates were separated by SDS/PAGE and transferred onto membrane. Membrane was probed with R525, E18, or E19. E18 and E19 did not bind denatured viral proteins and, therefore, recognize conformational epitopes. (B) Indirect ELISA was performed by coating wells with recombinant viral proteins or heat-inactivated EV71-infected cell lysates. Various concentrations of mAb were added in duplicates. The mAbs were detected by HRP assay (Materials and Methods). The amount of bound mAbs are presented as average OD₄₅₀ ± SDs.