Fig. 1.

(A) Heatmap representation of MIPE library activity derived from qHTS of a previously reported (28) NF-κB assay in agonist (increased transcriptional response) and antagonist (decreased transcriptional response) mode and cell viability (percent cells remaining); control viability in hMSC, TMD8, and HBL1 lines; apoptotic response in TMD8 cells at 8 and 16 h. Red represents inhibitory response; green represents activation response; color intensity represents potency (stronger intensity means lower EC₅₀ value). (B) Expansion of these data for selected agents of interest. (C) Complete response curves from these data sets for the CDK 1–5 inhibitor PHA-793887. [○, NF-κB activation; x, NF-κB viability; □, hMSC cell viability; ■, TMD8 cell viability; ▲, TMD8 apoptotic response (8 h); △, TMD8 apoptotic response (16 h)].