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. 2014 Jan 27;111(6):2164–2169. doi: 10.1073/pnas.1318737111

Fig. 2.

Fig. 2.

C2cd3 is localized to the distal end of centrioles and is required for distal appendage assembly. (A) C2cd3 is localized distal to ninein on centrioles. On the mother centriole, ninein is localized to both the proximal end and subdistal appendages (SAs), appearing as three dots on a lateral view. Ninein is also localized to the proximal end of the daughter centriole, appearing as a single dot. The illustration schematizes the localization of C2cd3 and ninein on centrioles. (B) C2cd3 is at the same distal level as Cep164 on the centriole. Upper shows a lateral view of the mother centriole in which Cep164 appears as a bar. Lower shows a top view of the mother centriole in which Cep164 appears as a ring. Note that Cep164 is only localized to the distal appendages (DAs) of mother centriole, whereas C2cd3 is localized to both centrioles. The diagram shows the localization of C2cd3 and Cep164 on the centrioles. Endogenous and overexpressed (C) Cep164, (D) GFP–Sclt1, (E) GFP–Ccdc41, (F) GFP–Cep89, and (G) GFP–Fbf1 fail to be recruited to the mother centriole in the absence of C2cd3. The centrosomes are labeled with γ-tubulin, and the nuclei are visualized with DAPI. For quantitative analyses, SD is indicated. n = 3 independent experiments.