Fig. 5.

Epitope mapping of rTT.C-specific antibodies. The histogram summarizes the normalized signal of five rTT.C-specific antibodies binding to a peptide array scan of an rTT.C sequence. The peptide array contained 110 15-mer peptides having a 12-residue overlap and spanning the entire rTT.C sequence. A negative control using Herceptin resulted in very low signal across the peptide array and positive control spots (absorbed full-length TT and rTT.C proteins). Computational modeling of three of these antibodies with the experimentally defined epitope is shown in Fig. S7.