Fig. 5.

Plexin-B2–mediated RhoA signaling is required for development of the liver vasculature. (A) Immunofluorescence staining of adult liver using anti-Plexin-B2 and anti-CD31 antibodies. (Scale bar, 50 µm.) (B) H&E-stained sections derived from untreated liver (Upper) or regenerating liver 7 d after 2/3 partial hepatectomy (Lower). Arrows point to bile ducts. (Scale bars, 100 µm.) (C) 3D reconstructions of Z stacks of CD31-stained sections derived from untreated liver (Upper) or regenerating liver 7 d after 2/3 partial hepatectomy (Lower). (Scale bar, 50 µm.) (D) Intravital fluorescence microscopy pictures of adult livers. (Scale bar, 200 µm.)