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. 2014 Jan 24;111(6):2277–2282. doi: 10.1073/pnas.1323977111

Fig. 4.

Fig. 4.

FXR activation with GW4064 induced AQP2 expression in cultured primary IMCD cells. (A) RT-PCR analysis of FXRα and FXRβ mRNA expression in three preparations of cultured primary IMCD cells. (B) Western blot assay showing FXR protein expression in the nuclei of primary IMCD cells. (C) Nuclear localization of FXR (red) and AQP2 (green) in a primary IMCD cell. The nucleus was visualized by staining the cell with DAPI (blue). (D and E) Primary IMCD cells were treated with 2.5 μM GW4064 for the indicated time points. Both AQP2 mRNA (D) and protein (E) levels were induced by the GW4064 treatment. (F and G) GW4064 treatment for 6 h failed to induce AQP2 expression at both mRNA (F) and protein (G) levels in primary IMCD cells isolated from FXR−/− mice. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 vs. DMSO (D, n = 3) and FXR+/+ mice (F, n = 4).