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(A) Steady-state kinetics of LigAB with PCA: anaerobically purified (red circles) and aerobically purified (blue squares). (B) Steady-state kinetics of aerobically purified LigAB (blue squares) and the time dependent inactivation of aerobically purified enzyme after thawing (green triangles) at pH 7.5, 25 °C.
