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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Immunol. 2013 Nov 27;192(1):293–299. doi: 10.4049/jimmunol.1300453

Figure 1.

Figure 1

Delayed RSV clearance and impaired M282–90 epitope-specific CD8+ T cell responses in aged mice. Young (n=5) and aged (n=5) BALB/c mice were infected with 3 × 105 PFU of A2-line19F virus. (A) RSV titers in lungs of infected young and aged BALB/c mice at days 1, 2, 4, 6, 8, 10, 12, 16, and 20. The lungs were harvested at indicated time points, and infectious RSV was titrated by plaque assay. Significant differences were observed between young and aged mice marked by an asterisk at day 2, 8 and 10 p.i. The solid line represents the limit of detection (* = P × 0.05, ANOVA). (B) The lung lymphocytes were harvested at indicated time points from right lungs of the same mice. M282–90-specific tetramer positive cells were quantified as a percentage of CD3+CD8+ T cells by flow cytometry. Data show an average of three separate experiments with equivalent results.