Figure 5.

Enumeration of CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺ , Tet⁺CD8⁺ T lymphocytes in blood. Groups of aged BALB/c mice (n=5) were vaccinated with TriVax + isotype control (iso, upper plots) or TriVax + α-41BB mAb (α-41BB, bottom plots) as illustrated in Fig. 4. PBLs were isolated at day 20. Polygon gates showed the % CD3⁺ T cells in blood. % CD3⁺CD4⁺ or % CD3⁺CD8⁺ T cells was shown in a rectangular gate in the middle plot. Upper right quadrant gates represented the % of CD3⁺CD8⁺ T cells that were M2_82–90 tetramer positive. Dot plots presented in Fig. 5 are representative of the percentage of CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, and CD8⁺Tet⁺ T cells.