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Expression analysis of caspase-3 in the brain. (A) Quantitative PCR analysis of caspase-3 in guinea pig foetal brains comparing vitC deficient (dark bars) to controls (light bars) on two gestational times points (GD45 and 56). Expressed values are normalized to the reference gene s18 and displayed as mean±SD. (B) Densitometric analysis of levels of cleaved caspase-3 obtained by Western blot and normalized to actin levels. Data are expressed as means±SD. ⁎p<0.05 by ANOVA.
