Fig. 7.

Dose dependent changes in mtDNA integrity and copy numbers following peroxynitrite treatment. (A) Representative PCR gel showing increases in long range (LR) mtDNA product (integrity) and short fragments (D-Loop and ND4; mtDNA copy number) following treatment with peroxynitrite (ONOO) (0–50 µM). Cells were treated with ONOO for 10 min, washed, and harvested 24 h later. β-Actin was used as a nuclear encoded control in the PCR reactions. (B) Graphs represent values after densitometric quantification of agarose gel results. All data shown are mean±SEM (n=7). *p<0.05 compared to control cells.