FIG. 2.

BrdU incorporation and cell viability assessment of Und-rASCs and Epith-rASCs after 48 h incubation with BrdU. (A) Immunofluorescent staining of the Und-rASCs and Epith-rASCs after labeling with BrdU. The Und-rASCs and Epith-rASCs (labeled with cytokeratin 19) colocalized with BrdU expression. Cytokeratin 19: green; nuclei of all the cells stained with Hoechst 33258: blue; nuclei of BrdU-labeled cells: red. Scale bars: 50 μm. (B) Proliferation of rASCs before and after labeling with BrdU, determined by DNA assay using Hoechst 33258. BrdU, 5-bromo-2′-deoxyuridine; Und-rASCs, Und-rASCs before labeling with BrdU; BrdU⁺ Und-rASCs, Und-rASCs after labeling with BrdU; Epith-rASCs, Epith-rASCs before labeling with BrdU; BrdU⁺ Epith-rASCs, Epith-rASCs after labeling with BrdU; Und-rASCs, undifferentiated rASCs. Color images available online at www.liebertpub.com/tea