Table 5.

Differential Expression of Proteins Identified from Normal and Engineered Cartilage

Dot no.	Distinct peptides	Distinct summed MS/MS search score	AA% coverage	UniProt	Protein name	Functional classification
2	2	29.24	6	O11780	Transforming growth factor-beta-induced protein ig-h3 precursor	Collagen assembly protien/cell adhesion
4	9	141.89	17	P09571	Serotransferrin	Metal binding
5	2	29.09	4	P09571	Serotransferrin	Metal binding
6	2	32.6	8	Q5S1S4	Carbonic anhydrase 3	Metal binding
7	4	60.87	22	Q5S1S4	Carbonic anhydrase 3	Metal binding
9	3	44.46	21	P00761	Trypsin precursor	Enzyme
11	2	36.07	14	Q8MK67	Phosphatidylethanolamine-binding protein 1	Binding protein/ATP binding
15	2	29.8	1	P28481	Collagen alpha-1(II) chain precursor	Structural constituent
17	3	50.06	19	P51779	Complement factor D precursor	Enzyme/immunity
18	2	41.26	7	Q1KYT0	Beta-enolase	Glycolysis
	4	76.87	16	Q9XSJ4	Alpha-enolase	Glycolysis
	2	40.29	9	P42897	Enolase	Glycolysis
20	6	104.71	14	P19120	Heat shock cognate 71 kDa protein	Transcription/ATP binding
	2	36.64	4	Q6BZH1	78 kDa glucose-regulated protein homolog precursor	Protein assembly/ATP binding
	2	33.46	4	P08108	Heat shock cognate 70 kDa protein	Protein assembly/ATP binding
	2	32.89	3	P08418	Heat shock 70 kDa protein homolog	Protein assembly/ATP binding
	2	31.99	3	P29843	Heat shock 70 kDa protein cognate 1	Microtubule assembly
	2	34.06	12	P00761	Trypsin precursor	Enzyme
21	2	31.19	7	Q1KYT0	Beta-enolase	Glycolysis
	4	66.94	14	Q9XSJ4	Alpha-enolase	Glycolysis
	3	52.57	11	P42897	Enolase	Glycolysis
	3	46.55	9	Q9W7L1	Alpha-enolase	glycolysis
	2	32.18	6	O57391	Gamma-enolase	Glycolysis
22	8	136.92	24	P79385	Lactadherin	Cell adhesion/angiogenesis
	3	54.87	11	P19140	Alpha-enolase	Glycolysis
	2	39.83	9	P42897	Enolase	Glycolysis
	2	33.93	6	Q05524	Alpha-enolase, lung specific	Glycolysis
23	3	52.25	13	P19620	Annexin A2	Metal binding
	2	31.67	10	O62823	Alpha-S1-casein precursor	Antioxidant
24	2	25.32	8	P20112	SPARC precursor	Angiogenesis/sinal transduction
25	2	27.35	5	P20305	Gelsolin precursor	Actin binding protein/apoptosis
27	5	80.14	19	O15335	Chondroadherin precursor	Collagen assembly protien
31	3	56.56	26	P80031	Glutathione S-transferase P	Metabolism
32	2	30.37	1	P20908	Collagen alpha-1(V) chain precursor	Structural constituent
33	2	31.47	12	Q9GKQ6	Biglycan precursor	Collagen fiber assembly
	2	41.81	1	P05539	Collagen alpha-1(II) chain precursor	Structural constituent
35	3	57.51	16	Q29371	Triosephosphate isomerase	Glycolysis
36	2	33.89	3	P08835	Serum albumin precursor	Metal binding/apoptosis
38	3	50.62	12	P79385	Lactadherin	Cell adhesion/angiogenesis
39	4	54.87	8	P30101	Protein disulfide-isomerase A3 precursor	Antioxidant/metabolism/apoptosis
	2	23.89	20	P81605	Dermcidin precursor	Antimicrobial activity
40	7	120.33	33	P08758	Annexin A5	Ca2 mediator/anti-apoptosis
	2	32.13	7	O15335	Chondroadherin precursor	Collagen assembly protien