Table 2.
Primers and Restriction Enzymes Used in the Analyses
| Disease | Mutation site | Primers | Restriction enzyme |
|---|---|---|---|
| HMSNL | 564C→T | HMSNL F: 5′-AAC TGT GGA GAA TAC GGG-3′ | TaqI |
| HMSNL R: 5′-CTG TGC AGG CAG TTA CGG CAG C-3′ | |||
| GM1 | 176G→A | GM1 F: 5′-CAT CCT TGC TCT GTA GAA TG-3′ | NlaIII |
| GM1 R: 5′-ACA GTT GTA TCT TCT CTC CAG-3′ | |||
| CCFDN | IVS6+389C→T | CCFDN F: 5′-CTT CCA GAG TTC ACG CCA TT-3′ | NlaIII |
| CCFDN R: 5′-ACA CAA AAG CCC AGC TCA AG-3′ | |||
| LGMD2C | 848G→A | LGMD2C F: 5′-CCT GTC TGT GGC CGG TGT GA-3′ | RsaI |
| LGMD2C R: 5′-GCG TTT ACT TCC CAT CCA CGC TGC-3′ |