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. 2013 Nov 11;20(3-4):518–528. doi: 10.1089/ten.tea.2013.0092

FIG. 3.

FIG. 3.

In vitro evaluation of neurite outgrowth and Schwann cell proliferation and extension on two-dimensional (2D) films of E10-0.5(1K) and PE in comparison with control substrates, tissue culture polystyrene (TCPS), and glass. (A) Neurite outgrowth distribution of rat spinal cord motor neurons on polymer-coated glass coverslips precoated with poly-L-lysine (PLL) and laminin. Neurites were binned based on their length. Bar height shift to right indicates longer neurite extensions on E10-0.5(1K)-coated coverslips as compared to PE and glass coverslips. Significantly, more neurite extensions reached 100 μm on E10-0.5(1K)-coated coverslips as compared to neurite extensions on PE and glass coverslips. (B) Schwann cell density on polymer-coated glass coverslips precoated with PLL. Significantly less Schwann cells attached to PE substrates as compared to TCPS, glass, and E10-0.5(1K) at 24 h. (C) Schwann cell extension length (μm) on polymer-coated glass coverslips precoated with PLL. Schwann cells exhibited greater extension on TCPS and E10-0.5(1K) substrates as compared to PE at 24 h. (*p<0.05, one-way analysis of variance with Tukey's post hoc test).