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Immunofluorescent staining for the marker of the undifferentiated pluripotent state of hESCs, Oct-3/4 (green); for the marker of early differentiating migrating hESC-derived cells, vimentin (red); and for all cell nuclei (DAPI, blue). The differentiating hESCs lose Oct-3/4 expression and become increasingly vimentin immunopositive by as early as the first passage (“hESC-D-P1”). Color images available online at www.liebertpub.com/tea
