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. 2014 Jan 24;20(3-4):740–750. doi: 10.1089/ten.tea.2012.0738

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 4. — Blood vessels engineered from differentiated hES-MSCs in a pulsatile bioreactor for 8 weeks. (A) Hematoxylin and eosin; (B) Masson's trichrome staining; (C) PCNA staining; (D–G) immunofluorescent staining of engineered tissue. (H) PCR profiles of engineered tissue (MSCs and SMCs cultured in vitro before bioreactor culture, V449- and V450-engineered tissue from separate experiments) for genes (a) αSMA, (b) CNN1, (c) SM22α, (d) PPAR, (e) OCN, and (f) COL2A. PCNA, proliferating cell nuclear antigen.