FIG. 1.

Seeding of human pluripotent stem cells (hPSCs) on Matrigel-coated microcarriers. (A) Efficiencies as fractions of seeded cells attached on the microcarrier surface are shown for seeded dispersed hPSCs or clusters. (B) Fraction of beads colonized after being seeded with dispersed cells or clusters. H9 human embryonic stem cells (hESCs) on Matrigel-coated beads stained with FDA after being seeded as (C) single cells or (D) small clusters. Microcarriers loaded with dispersed cells are shown at days 0, 3, and 6. Stars mark microcarriers with only a few or no cells. Scale bars: 200 μm. (E) Expansion of hESCs in spinner flasks after their seeding on Matrigel-coated microcarriers and 4 h (dashed curve) or overnight (solid curve) treatment with ROCK inhibitor. [^#p<0.05 in (A, B).] Color images available online at www.liebertpub.com/tea