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Multi-passage expansion of H9 hESCs on Matrigel-coated microcarriers. (A) Growth profile and viability of microcarrier-attached cells cultured for five passages. (B) Corresponding cumulative lactate dehydrogenase (LDH) activity. Cells were probed for the expression of pluripotency markers by (C) quantitative polymerase chain reaction (qPCR), (D) flow cytometry, and (E) immunostaining (scale bars: 50 μm). Color images available online at www.liebertpub.com/tea
