FIG. 5.

Human PSCs cultured for multiple passages on CP+pLL microcarriers in stirred-suspension vessels. (A) Growth profile, viability, and (B) cumulative LDH activity of IMR90 hiPSCs cultured for five passages on microcarriers in spinner flasks. After each passage, the expression of pluripotency markers was characterized by (C) qPCR and (D) flow cytometry. (E) After the last passage, cells were plated and stained for pluripotency markers (scale bars: 50 μm). (F) Karyotyping results for IMR90 cells after their culture for five passages on CP+pLL beads in spinner flasks. Color images available online at www.liebertpub.com/tea