FIG. 6.

Differentiation potential of hPSCs after their propagation on CP+pLL microcarriers for five passages. (A) IMR90 hiPSCs were subsequently cultured as embryoid bodies and expression of markers of the three embryonic germ layers was assessed. The expression of undifferentiated IMR90 cells was used for normalization. Alternatively, propagated cells were plated and subjected to directed differentiation toward (B, E) definitive endoderm (DE), (C, F) mesoderm, and (D, G) neuroectoderm. The expression was evaluated by qPCR (B–D) and immunostaining (E–G). Gene expression was normalized to IMR90 cells differentiated in basal medium without differentiation factors. Scale bars in (E–G): 50 μm. Color images available online at www.liebertpub.com/tea