FIG. 7.

Directed differentiation of hPSCs cultured on CP+pLL microcarriers in a stirred-suspension bioreactor. (A) Expression of MS genes after IMR90 hiPSC differentiation in the bioreactor. Gene expression was normalized to hiPSCs differentiated in basal medium without factors. (B) Immunostaining of differentiated cells on beads. Stars denote the position of microcarriers. Scale bars: 100 μm. (C) Flow cytometric analysis of KDR expression. A flow cytometry graph is shown from a representative experiment. Curves correspond to samples of negative control (black), cells in dishes incubated in basal media without differentiation factors (green; dish control), cells differentiated in dishes (red) and cells differentiated in microcarrier suspension culture (blue). The gate excludes 99% of the negative control cells. Results are summarized in the bar graph (n=3) as mean±SD.