Young's modulus of gel disks as a function of the applied LVG alginate concentration (A). The effect on Young's modulus of gel disks added 1.0% or 0.5% LVG alginate followed by immersion in isotonic gelling solutions of either SrCl2 or CaCl2
(B). Young's modulus over time for gel disks-added 0.5% LVG alginate and kept in Dulbecco's-modified Eagle's medium (DMEM), which was replaced thrice per week (C). Characterization of gel disks in A, B, and C (Day 0) was accomplished on disks kept in DMEM for 2 h. Significance is considered between different treatments for the same concentration of added alginate. *p<0.05, **p<0.01, and ***p<0.001. Error bars present SD.