FIG. 4.

Distribution of NIH:3T3 cells in gel disks and cell seeding efficiency influenced by the alginate concentration in the cell seeding solution 2 h after seeding. Cell localization throughout the thickness of the gel indicated by colors when cells were suspended in cell seeding solutions containing alginate concentrations of 0% (A), 0.1% (B), 0.5% (C), and 1.0% (D). Cell distribution in gels showed for xy- and xz-directions (E). Percent cell seeding efficiency as a function of alginate concentration in the cell seeding solution (F). Magnification: ×40. Scale bars: 100 μm, Color bars: foam thickness 0–400 μm (A), 0–700 μm (B, D) and 0–600 μm (C). The error bars depict SD.