FIG. 3.

Non-neural alignment and number poststimulation. (A) The frequency distribution of Schwann cell orientation measured using ImageJ after 12 h poststimulation exhibits maximal bias at 50 mV/mm parallel to the previously generated electric field, while endothelial cells and fibroblasts do not exhibit a directional bias. 90° represents the direction of the previously applied electric field. (B) At 50 mV/mm, no significant changes were observed in cell density of stimulated Schwann cells (SC), endothelial cells (EC), and fibroblasts (FB) in comparison to unstimulated paired controls. Thresholded and binarized cell nuclei were counted before analysis of major angle for each 10× image captured. n=3, standard deviation shown.