FIG. 3.

(A–H) Representative cross sections stained for CD31 (red) from the middle region of constructs from all days and flow conditions studied. PCs were GFP-labeled (green), and nuclei were stained with Hoechst 33342 (blue). Scale bars=50 μm. (I–N) Quantification of images. (I) The fraction of the section stained positively for CD31 remained constant across all compacted conditions. (J) The number of lumens per square millimeter was increased by low flow relative to time-matched controls but not by high flow. (K) The mean diameter of lumens decreased with compaction but was independent of day or flow condition. (L) The cell number per square millimeter, based on Hoechst 33342 staining, was somewhat variable between conditions, but no trends emerged. (M) The number of PCs per square millimeter was reduced with exposure to either flow rate. (N) The fraction of PCs that were recruited to CD31+ microvessels was the same across all conditions. *p<0.05. ^$p<0.05 in comparison with day 5 control. ⁺p<0.05 in comparison with day 8 control. Color images available online at www.liebertpub.com/tea