Skip to main content
. Author manuscript; available in PMC: 2014 Feb 17.
Published in final edited form as: Bioorg Med Chem Lett. 2011 Dec 7;22(2):1023–1026. doi: 10.1016/j.bmcl.2011.11.128

Figure 1.

Figure 1

Agonist-induced recruitment of βarr2-GFP to KOPR in U2OS cells. Live cell confocal imaging of KOPR-βarr2eGFP-U2OS cells reveals robust recruitment of β-arrestin 2 to the KOPR by salvA and its derivatives as evidenced by the formation of intense green punctae after 10 min of drug exposure. A 5× magnification of the region outlined by the white square in each image is shown on the right. SalvA and the two derivatives (1 and 3) (10 μM) robustly recruit β-arrestin 2 to the KOPR (bright punctae), while nalbuphine (10 μM), a partial agonist, recruits less βarr2-GFP. Similar results obtained from three independent experiments performed on separate days, representative images shown.