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Western blot detection of connexin 43 (Panel A) and connexin 50 (Panel B) in freshly isolated porcine NPE and in cultured porcine NPE. Glutathione-S-transferase fusion protein containing the cytoplasmic carboxy-terminal tail of Connexin 43 (GST-Cx43) and porcine lens fiber were used as positive controls for connexin 43 and connexin 50, respectively. 60 μg of protein was loaded to each lane.
