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Thapsigargin induces cell apoptosis in A549 cells. (a) Hoechst staining (blue) showing the increasing of apoptotic A549 cells by thapsigargin (1 nM, 100 nM, and 1 μM) treatment for 6 and 24 h (white arrows). (b) Histograms showing the quantification of the cell death (%) in A549 cells after thapsigargin treatment. Results are averages of three independent experiments. Data represent mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001.
