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. 2014 Feb 11;13:22. doi: 10.1186/1475-2859-13-22

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Copyright © 2014 Čiplys et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Secretion of human BiP protein into the yeast growth medium. SDS-PAGE (A) and Western blot against human BiP protein (B) of crude growth medium (40× concentrated) of yeast cells carrying control vector pFDC or pFDC-BiP for expression of human BiP. Cells were incubated for 36 h in YEPD medium. M – prestained molecular ladder (ThermoScientific, cat. no. 26618).