Figure 2.

Archival outperforms Modern AM1 under standard growth conditions. A) Representative growth curves showing the increase in OD₆₀₀ over time of Modern (black circles) and Archival AM1 (gray squares) cultured using 3.5 mM succinate in 48-well plates. B) Growth rates calculated from the exponential phase of cultures grown on methanol (M), methylamine (Ma), or succinate (S) as a carbon source. Significant growth differences between Modern and Archival were calculated using a two-tailed, unpaired t test, and are marked by single (p < 0.05) and double asterisks (p < 0.01). C) Fitness of Archival AM1 measured via a head-to-head competition mixed in co-culture with a fluorescently labeled Modern reference. A control growth consisted of unlabeled Modern (black) versus the fluorescent Modern reference grown on M. All other bars (gray) show Archival fitness relative to Modern grown M, Ma, and S. Values are the mean plus SEM of growth rates or fitness values calculated from three or more biological replicates (see Methods).