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. Author manuscript; available in PMC: 2014 Feb 17.
Published in final edited form as: Biochem Biophys Res Commun. 2009 May 3;384(3):280–283. doi: 10.1016/j.bbrc.2009.04.107

Fig. 1.

Fig. 1

The Drosophila retinoblastoma tumor suppressor colocalizes with the spliceosomal component SmD3 in salivary gland nuclei. The amino-terminal domain of Drosophila Rbf fused to RFP (RbfN-RFP) colocalizes with a GFP-tagged SmD3 in larval salivary gland cells. The DNA stain DAPI was used to show the bands of the characteristic polytene chromosomes. Confocal imaging shows extensive colocalization of SmD3-GFP and Rbf1N-RFP in the nucleoplasm and along the chromsomes. Salivary glands were isolated from mature larvae and fixed with 4% formaldehyde. Confocal images were obtained using a Zeiss LSM510 Meta.