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. Author manuscript; available in PMC: 2014 Feb 17.
Published in final edited form as: Methods Enzymol. 2012;512:293–314. doi: 10.1016/B978-0-12-391940-3.00013-5

Figure 5.

Figure 5

DNase I footprinting of the EC-5 and EC+41 formed by E. coli RNAP on the 603-42 DNA and nucleosomal templates. The promoter end-labeled DNA and nucleosomal templates were incubated in the presence of DNase I and separated by native PAGE (Fig. 4). DNA from different bands was gel-purified and separated by denaturing PAGE. The regions occupied by RNAP in the EC-5 and EC+41 are indicated by dashed lines. The position of the labeled DNA end is indicated by asterisk. M – end-labeled pBR322-MspI digest.