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Substitution of the β-strand “stalk” in bovine antibody BLV1H12 with a coiled-coil motif. (A) X-ray crystal structure of antibody BLV1H12 (PDB ID: 4K3D). (B) Helical wheel representation of the antiparallel heterodimeric coiled-coil.[8] The sequence of the ascending peptide with linkers at each end is: H2N-GGSGAKLAALKAKLAALKGGGGS-COOH; the sequence of the descending peptide with linkers at each end is: H2N-GGGGSELAALEAELAALEAGGSG-COOH.
