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. Author manuscript; available in PMC: 2015 Jan 3.
Published in final edited form as: Angew Chem Int Ed Engl. 2013 Nov 19;53(1):132–135. doi: 10.1002/anie.201307939

Figure 2.

Figure 2

Hydrogen deuterium exchange mass spectrometry (HDX-MS) analysis of Ab-coil Fab, Base peptide alone, and the Acid-Base complex. Antibody or peptide sample was diluted into D2O-containing exchange buffer (50 mM HEPES, pH 8.0, 150 mM NaCl) and incubated at 4°C for 10 to 3600 s. Measurements were repeated three times; all values were calculated based on experimental Dmax values. (A) Deuterium incorporation curves of the backbone amides within the coiled-coil regions of Ab-coil Fab and the Base peptide as measured by liquid chromatography-mass spectrometry (LC-MS) at multiple time points. (B) The average levels of deuterium incorporation for the coiled-coil regions of Ab-coil Fab, Base peptide alone, and the Acid-Base complex. The value within each peptide is the average % of deuterium incorporation over 6 time points (from 10 to 3600 s). The numbers in the parentheses are standard deviation and charge state of the analyzed peptide.