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. Author manuscript; available in PMC: 2015 Jan 3.
Published in final edited form as: Angew Chem Int Ed Engl. 2013 Nov 19;53(1):132–135. doi: 10.1002/anie.201307939

Figure 3.

Figure 3

Ab-coil bGCSF fusion protein stimulates proliferation of mouse NFS-60 cells in a dose-dependent manner. Cells cultured in RPMI-1640 medium with 10% FBS and 0.05 mM 2-mercapoethanol were treated with various concentrations of bGCSF, Ab-beta IgG, Ab-coil IgG, Ab-beta bGCSF IgG and Ab-coil bGCSF IgG. Cell viability was quantified using an Alamar Blue (Invitrogen) assay; measurements were repeated three times.