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. 2014 Jan 10;14(1):1195–1207. doi: 10.3390/s140101195

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — (A) REEAD based detection of hTopI activity in whole cell extract of Caco2 and HT29 in the presence of CPT. One example (Raw data) randomly picked out of 30 individual microscopic images of each triplicate reaction sample is shown, red signals represent RCA products generated from circularized S(hTopI) (i.e., generated by hTopI activity) and green signals represent RCA products generated from control circles. (B) REEAD based quantification of CPT induced inhibition of hTopI activity in whole cell extract of Caco2 and HT29, depicted as mean ± SD in a log2 XY-plot. The data were collected from triplicate reactions, and each triplicate was normalized to the sample without CPT (thus DMSO only) so that the hTopI activity in the sample without CPT was set to one.